CD9 antibody | knockout validation | BD Pharmingen 555370

This is a knockout-validated antibody summary, based on the publication "The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD9 antibody | knockout validation | BD Pharmingen 555370 figure 1
Figure 1. Western blot analysis of 293T and HeLa clonal cell lines. Actin and the tetraspanin CD63 are used as loading controls.
Antibody information

Mouse monoclonal BALB/c IgG1, κ

Company: BD Pharmingen

Antibody: CD9

Catalog number: 555370

Summary: Mouse monoclonal IgG1, κ against human C-ALL cells (CD9 antigen). Reacts with human by western blot, flow cytometry, and immunohistochemistry (frozen). Not recommended for immunohistochemistry (paraffin).

CD9 antibody | knockout validation | BD Pharmingen 555370 figure 2
Figure 2. Immunofluorescent analysis of HeLa clonal cell lines. From [1].
Validation Method

Western blot | Immunocytochemistry

Sample

WT and CD9-KO 293T and HeLa clonal cell lines.

Secondary incubation

WB: goat-anti-mouse HRP conjugated antibodies (Thermo Scientific).

IF: goat-anti-mouse-AlexaFluor 488 or 568 antibodies (Invitrogen).

References
  1. Earnest J, Hantak M, Li K, McCray P, Perlman S, Gallagher T. The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases. PLoS Pathog. 2017;13:e1006546 pubmed publisher