CD81 antibody | knockout validation | BD Pharmingen 555675

This is a knockout-validated antibody summary, based on the publication "The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD81 antibody | knockout validation | BD Pharmingen 555675 figure 1
Figure 1. Western blot analysis of 293T and HeLa clonal cell lines. Actin and the tetraspanin CD63 are used as loading controls.
Antibody information

Mouse monoclonal IgG1, κ

Company: BD Pharmingen

Antibody: CD81

Catalog number: 555675

Summary: Mouse monoclonal IgG1, κ against human CD81. Reacts with human, Rhesus, Cynomolgus, baboon, dog, cat, rabbit, and horse. Suitable for flow cytometry.

CD81 antibody | knockout validation | BD Pharmingen 555675 figure 2
Figure 2. Immunofluorescent analysis of HeLa clonal cell lines. From [1].
Validation Method

Western blot | Immunofluorescence

Sample

WT and CD9-KO 293T and HeLa clonal cell lines.

Secondary incubation

WB: goat-anti-mouse HRP conjugated antibodies (Thermo Scientific).

IF: goat-anti-mouse-AlexaFluor 488 or 568 antibodies (Invitrogen).

References
  1. Earnest J, Hantak M, Li K, McCray P, Perlman S, Gallagher T. The tetraspanin CD9 facilitates MERS-coronavirus entry by scaffolding host cell receptors and proteases. PLoS Pathog. 2017;13:e1006546 pubmed publisher