This is a knockout-validated antibody summary, based on the publication "Retinal Pre-Conditioning by CD59a Knockout Protects against Light-Induced Photoreceptor Degeneration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Photomicrograph showing CD59a immunolabeling in all layers of mouse retina, RPE and choroid (B), but not in CD59aKO eyes (C). RPE, retinal pigment epithelium; OS, photoreceptor outer segment; IS, photoreceptor inner segment; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. From [1].

Mouse monoclonal IgG1

Company: Hycult

Antibody: CD59a

Catalog number: HM1116

Summary: Mouse monoclonal IgG1 against mouse CD59a-Fc. Reacts with mouse by western blot, immunofluorescence, immunohistochemistry (frozen), flow cytometry, immunoassays, functional studies, and flow cytometry.

Immunohistochemistry

WT and CD59aKO mouse retina. Eyes were immersion-fixed in 4% paraformaldehyde (PFA) for 10 min, infiltrated in 30% sucrose overnight and embedded in Tissue-Tek OCT (Sakura Finetek, Torrance, CA, USA) for sectioning.

Mouse-on-mouse kit (ab127055, Abcam, MA, USA).

1:500 dilution.

HRP-conjugated secondary, mouse-on-mouse polymer IHC kit (ab127055, Abcam, MA, USA).

Diaminobenzidine.

The same clone (7A6) is sold as Hycult Biotech HM1116, HM1116F; LifeSpan Biosciences LS-C210253, LS-B13137.