This is a knockout-validated antibody summary, based on the publication "CD56 regulates human NK cell cytotoxicity through Pyk2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative confocal microscopy images of WT or CD56-KO NK92 effectors conjugated to K562 target cells in the presence of non-blocking CD56 antibody then fixed and immunostained for perforin, pPyk2 Y402 and actin. From [1].

Mouse monoclonal IgG1,κ

Company: Biolegend

Antibody: CD56

Catalog number: 318314

Summary: Mouse monoclonal IgG1,κ against CD56. Conjugated with Alexa Fluor® 647. Reacts with human, African green, baboon, Cynomolgus, Rhesus. Not recommended for immunohistochemistry (paraffin).

Western blot

WT or CD56-KO NK92 cells.

1:100 dilution.

Primary antibody was Alexa Fluor 647 conjugated.

Images were acquired through a 63 × 1.40 NA objective on a Zeiss AxioObserver Z1 microscope stand equipped with a Yokogawa W1 spinning disk.

The same clone (HCD56) is sold as BioLegend 318302, 318303, 318304, 318305, 318306, 318308, 318309, 318310, 318311, 318312, 318313, 318314, 318315, 318316, 318317, 318318, 318319, 318320, 318321, 318322, 318324, 318325, 318326, 318327, 318331, 318332, 318329, 318333, 318328, 318335, 318339, 318341, 318342, 318340, 318334, 318336, 318343, 318345, 318347, 318348, 318330, 318344; LifeSpan Biosciences LS-B1881; Abnova MAB12531.