CD55 antibody | knockout validation | Santa Cruz sc-51733

This is a knockout-validated antibody summary, based on the publication "Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD55 antibody | knockout validation | Santa Cruz sc-51733 figure 1
Figure 1. Western blot analysis of WT TIME cells and KO cells ± cDNA. b-Actin was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: CD55

Catalog number: sc-51733

Summary: Mouse monoclonal antibody against human CD55 (DAF). Recommended for detection of CD55 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), and flow cytometry.

Validation Method

Western blot

Sample

Control and DAF KO TIME cells.

Primary incubation

1:200 dilution.

Secondary incubation

1:10,000 dilution IRDye 680LT Goat α-Mouse secondaries Abs (LI-COR).

Detection

LI-COR Fc fluorescent imager.

Clone note

The same clone (NaM16-4D3) is sold as OriGene AM39023RP-N; Santa Cruz Biotechnology sc-51733.

References
  1. Dieterle M, Solà Riera C, Ye C, Goodfellow S, Mittler E, Kasikci E, et al. Genetic depletion studies inform receptor usage by virulent hantaviruses in human endothelial cells. elife. 2021;10: pubmed publisher