This is a knockout-validated antibody summary, based on the publication "CD47-blocking antibodies restore phagocytosis and prevent atherosclerosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Company: Novus

Antibody: CD47

Catalog number: NBP2-31106

Summary: Mouse monoclonal IgG1 against human CD47. Reacts with human by western blot, immunoprecipitation, immunohistochemistry and flow cytometry.

Western blot

WT and CD47−/− mice tissues. Total protein was isolated from tissue homogenates using 1X cell lysis Buffer (Cell Signaling) supplemented with 1X Halt Protease & Phosphatase Single-Use Inhibitor Cocktail (Thermo Scientific).

5% bovine albumin serum solution prepared in 1X TBST for 1h.

1:1,000 dilution overnight at 4°C.

1:5,000 dilution HRP-conjugated anti mouse antibodies (Life Technologies) for 1 h.

SuperSignal West Pico Chemiluminescent substrate (Thermo Scientific).

Western blotting of tissue homogenates obtained from WT and CD47−/− mice (with and without quenching CD47 peptide) confirms the specificity of the antibody. Please see Extended Data Figure 2b in the article [1].

The same clone (B6H12.2) is sold as Novus Biologicals NBP2-31106; LifeSpan Biosciences LS-C87683, LS-C87684, LS-C148708, LS-C357794; Abnova MAB11342; Rockland Immunochemicals 200-302-M68, 200-308-M68; Invitrogen MA5-11895.