This is a knockout-validated antibody summary, based on the publication "Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2A
Company: R&D systems
Antibody: CD44s
Catalog number: BBA10
Summary: Mouse monoclonal IgG2A against a recombinant human CD44v3-10 (includes the invariant N-terminal exons and CD44v3-10 exons). Reacts with human by western blot, immunohistochemistry, immunoprecipitation and flow cytometry. This antibody recognizes an epitope in the invariant N-terminal region of all CD44 protein isoforms.
Western blot
Control, CD44-knocked out (K.O.), and CD44-reconstituted HeLa cells.
1:500 dilution.
Horseradish peroxidase conjugated secondary (1:10,000; Santa Cruz Biotechnology, Cat# sc-2005 or 1:100,000; EMD Millipore, Cat# AP130P).
SuperSignal West Pico chemiluminescent substrate (Pierce) or SuperSignal West Femto chemiluminescent substrate (Pierce).
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