This is a knockout-validated antibody summary, based on the publication "Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: DSHB
Antibody: CD44
Catalog number: 5D2-27
Summary: Rat monoclonal IgG2a against NIH 3T3 fibroblast plasma membranes. Reacts with mouse by western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, flow cytometry, and ELISA.
Western blot
Wild-type or CD44−/− murine BMDM.
Appropriate secondary antibodies conjugated to HRP (Jackson ImmunoResearch).
Chemiluminescence (Amersham ECL prime, GE Healthcare) on an Odyssey FC Imaging System (LI-COR) equipped with Image Studio Software (Li-COR).
CD44 expression in wild-type or CD44−/− murine BMDM. Please see Figure 2b in the article [1].
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