This is a knockout-validated antibody summary, based on the publication "Epidermal-specific deletion of CD44 reveals a function in keratinocytes in response to mechanical stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. A pan CD44 antibody (IM7; red, green) was used for indirect immune fluorescence staining of skin sections or keratinocytes (lower panel) harvested from Cd44Δker mice or control animals. Nuclei were counterstained with DAPI (blue). White arrows indicate areas where CD44 is removed (epidermis, hair follicles). From [1].

Rat monoclonal IgG2b

Company: BD Biosciences

Antibody: CD44

Catalog number: 553131

Summary: Rat monoclonal IgG2b against dexamethasone-induced SJL mouse spontaneous myeloid leukemia M1 cells. Reacts with mouse by flow cytometry,

immunoprecipitation, ELISA, radioimmunoassay, immunohistochemistry (paraffin and frozen), cytotoxicity, blocking-activation assays.

Immunohistochemistry | Immunocytochemistry

Skin sections (frozen or paraffin) and keratinocytes from control and Cd44Δker mice.

5% FCS in PBS for 1 h at room temperature.

2 μg/ml in blocking solution overnight.

1:500 dilution Alexa Fluor labeled secondary antibodies in blocking solution for 1 h at room temperature.

Antibodies pan CD44 antibody (KM201) or a CD44v6 specific antibody (α9A4) are KO validated by IHC and WB (Fig 1b,c), but Authors do not specify the manufacturer in the article. The author indicated that the KM201 Ab was from Abcam (ab25340). The 9A4 is prepared by authors. The hybridoma cells 9A4 can be found in ATCC.

The same clone (IM7) is sold as Bio-Rad MCA4703, MCA4703F, MCA4703PE; BioLegend 103003, 103004, 103009, 103010, 103011, 103012, 103015, 103016, 103017, 103018, 103019, 103020, 103025, 103026, 103027, 103028, 103029, 103030, 103031, 103032, 103035, 103036, 103039, 103037, 103041, 103043, 103046, 103047, 103049, 103051, 103054, 103001, 103002, 103005, 103006, 103007, 103008, 103013, 103014, 103021, 103022, 103023, 103024, 103033, 103055, 103056, 103057, 103059, 103061, 103062, 103040, 103044; OriGene TA320281; Santa Cruz Biotechnology sc-18849; LifeSpan Biosciences LS-C105865, LS-C106745, LS-C106844, LS-C107269, LS-B5508, LS-B9911; Abnova MAB11069; Invitrogen 11-0441-81, 11-0441-82, 11-0441-85, 11-0441-86, 12-0441-81, 12-0441-82, 12-0441-83, 13-0441-81, 13-0441-82, 13-0441-85, 13-0441-86, 14-0441-81, 14-0441-82, 14-0441-85, 14-0441-86, 15-0441-81, 15-0441-82, 15-0441-83, 16-0441-81, 16-0441-82, 16-0441-85, 16-0441-86, 17-0441-81, 17-0441-82, 17-0441-83, 25-0441-81, 25-0441-82, 45-0441-80, 45-0441-82, 47-0441-80, 47-0441-82, 48-0441-80, 48-0441-82, 56-0441-80, 56-0441-82, MA1-10225, MA1-10229, 61-0441-80, 61-0441-82, 83-0441-41, 83-0441-42, 67-0441-82, 62-0441-82, 67-0441-80, 62-0441-80, 64-0441-82, 64-0441-80, 63-0441-80, 63-0441-82; Bio X Cell BE0039-5MG; Sigma-Aldrich SAB4700188, SAB4700189; BD Biosciences 553134, 553132, 550538, 553131, 559250, 553130, 553135, 553133, 561860, 561862, 562464, 561859, 561861, 560781, 560780, 560570, 560569, 560568, 560567, 563114, 563058, 563736, 563970, 563971, 560452, 560451; EMD Millipore MABF423, MABF424, MABF425, MABF426, MABF427, MABF580, MABF581, MABF582, MABF583, MABF584.