CD44 antibody | knockout validation | Abcam ab51037

This is a knockout-validated antibody summary, based on the publication "Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD44 antibody | knockout validation | Abcam ab51037 figure 1
Figure 1. CD44 expression profile in WT but not in CD44 KO hNSC was verified by western blot. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CD44

Catalog number: ab51037

Summary: Rabbit monoclonal antibody against a synthetic peptide of human CD44. Reacts with human. Does not react with mouse or rat. Suitable for western blot, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.

Validation Method

Western blot | Immunocytochemistry

Sample

CD44 WT and KO human neural stem cells (hNSC).

Primary incubation

WB: 1:1,000 dilution.

ICC: 1:500 dilution.

Secondary incubation

WB: 1: 5,000 dilution ECL donkey anti-rabbit IgG, HRP-linked whole Ab.

ICC: 1: 1,000 dilution Alexa Fluor 555-conjugated donkey anti-rabbit.

References
  1. Benavente F, Piltti K, Hooshmand M, Nava A, Lakatos A, Feld B, et al. Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair. elife. 2020;9: pubmed publisher