CD38 antibody | knockout validation | BD Biosciences 611114

This is a knockout-validated antibody summary, based on the publication "Probing the requirement for CD38 in retinoic acid-induced HL-60 cell differentiation with a small molecule dimerizer and genetic knockout", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

CD38 antibody | knockout validation | BD Biosciences 611114 figure 1

Figure 1. Western blot of CD38 in wild-type and CRISPR-derived cell lines after treatment with 1μM RA as indicated. From [1].

Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: CD38

Catalog number: 611114

Summary: Mouse monoclonal IgG1 against human CD38 (aa. 171-292). Reacts with human by western blot and immunofluorescence.

Validation Method

Western blot

Sample

Wild-type and CD38-KO HL-60 cell lines. Cells were lysed with ice cold M-PER Mammalian Protein Extraction Reagent (Pierce, Rockford, IL) with protease and phosphatase inhibitor cocktails (Sigma, St. Louis, MO).

Blocking agent

5% dry nonfat milk in PBS.

Secondary incubation

HRP-linked anti-mouse (7076 S, Cell Signaling Technologies (Danvers, MA)).

Detection

Bio-Rad VersaDoc MP 5000.

References

Macdonald R, Shrimp J, Jiang H, Zhang L, Lin H, Yen A. Probing the requirement for CD38 in retinoic acid-induced HL-60 cell differentiation with a small molecule dimerizer and genetic knockout.. Sci Rep. 2017;7:17406 pubmed publisher

contribute

If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.