CD36 antibody | knockout validation | Cascade ABM-5525
DOI
//dx.doi.org/10.13070/ko.en.7.2213
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD36 antibody | knockout validation | Cascade ABM-5525 figure 1
Figure 1. Western blot of CD36 in Lean, Lepob/ob and Lepob/ob CD36-/- mice hearts (n = 6 per group). β-actin was used as loading control. From [1].
Antibody information

Mouse monoclonal IgA

Company: Cascade

Antibody: CD36

Catalog number: ABM-5525

Summary: Mouse monoclonal IgA against full-length adenovirus vector injected into CD36 null mice. Reacts with rat and mouse. Suitable for immunofluorescence, immunoprecipitation, flow cytometry and functional studies.

Validation Method

Western blot

Sample

Lepob/+ control (Lean), Lepob/ob (Lep null) and Lepob/ob CD36-/- (double null) mice hearts.

Blocking agent

5% nonfat dry milk in Tris-buffered saline with 0.1% Triton X-100.

Primary incubation

1:1,000 dilution.

Secondary incubation

Polyclonal rabbit anti-mouse IgG, peroxidase conjugated (sc-358923, Santa Cruz Biotech.).

Detection

ECL Plus (Pierce, Rockford, IL) and Hyperfilms.

References
  1. Gharib M, Tao H, Fungwe T, Hajri T. Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart. PLoS ONE. 2016;11:e0155611 pubmed publisher