This is a knockout-validated antibody summary, based on the publication "CD36 is involved in oleic acid detection by the murine olfactory system", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (E) Confocal image of a cryosection (P8) immunostained for CD36 (green) and the mature olfactory neuron marker OMP (red) in CD36−/− mice, showing absence of labeling. (F,G) Single channels of the picture shown in (E). From [1].

Rat monoclonal IgG2a

Company: AbD Serotec

Antibody: CD36

Catalog number: MCA2748

Summary: Rat monoclonal IgG2a raised against IL-4 treated murine thioglycollate-elicited peritoneal macrophages. It detects mouse CD36 protein recommended for flow cytometry and immunofluorescence.

Immunohistochemistry

Head sections (olfactory epithelium) of CD36−/− mice. The head was fixed for 2 h at 4°C in fixative solution, cryoprotected in 30% sucrose, and embedded in Leica tissue freezing medium (Leica Microsystems). Sections of 12 μm thickness were cut on a Leica CM 1900 UV cryostat (Leica Microsystems), adhered to Super Frost® Plus cryoslides (Thermo Scientific) and stored at −25°C.

1x PBS+/+ (PBS + 0.9 mM CaCl2, 0.48 mM MgCl2), 0.1% Triton X-100, 1% gelatin (Sigma, G7765) for at least 1 h

1:200, overnight at 4°C

1:500 secondary antibody for 1 h

confocal microscopy with TCS SPE and TCS SP5 systems (Leica microsystems)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

The same clone (MF3) is sold as Bio-Rad MCA2748A488, MCA2748F, MCA2748EL, MCA2748, MCA2748GA, MCA2748PE, MCA2748A647; LifeSpan Biosciences LS-C188398, LS-C188399, LS-C188400, LS-C188401; Invitrogen MA5-16831, MA5-16832, MA5-16833.