CD29 antibody | knockout validation | BD Biosciences 610467

This is a knockout-validated antibody summary, based on the publication "Novel ecto-tagged integrins reveal their trafficking in live cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot detection of endogenous and ectopic β1 integrins in control fl/fl and KO fibroblasts using a pan-β1 integrin antibody (top panel) and an anti-GFP antibody (middle panel). Tubulin was used as a loading control (bottom panel). From [1].

Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: CD29

Catalog number: 610467

Summary: Mouse monoclonal IgG1 against human CD29( aa. 76-256). Reacts with human, mouse, rat, dog, and chicken. Suitable for western blot, immunohistochemistry-formalin (antigen retrieval required), immunoprecipitation and immunofluorescence.

Validation Method

Western blot

Sample

Control fl/fl and β1 integrin KO mouse fibroblasts.

Primary incubation

1:1,000 dilution.

Secondary incubation

IRDye680 or IRDye800-conjugated secondary antibodies.

Detection

Odyssey CLx Imaging system.

References

Huet Calderwood C, Rivera Molina F, Iwamoto D, Kromann E, Toomre D, Calderwood D. Novel ecto-tagged integrins reveal their trafficking in live cells. Nat Commun. 2017;8:570 pubmed publisher

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