This is a knockout-validated antibody summary, based on the publication "CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1, kappa

Company: eBiosciences

Antibody: CD274 (PD-L1, B7-H1)

Catalog number: 14-5983-82

Summary: Mouse monoclonal IgG1, kappa against human B7-H1, also known as PD-L1. Reacts with human. It has been published to react also with dog, and mouse. Suitable for western blot, flow cytometry, and immunohistochemistry (frozen).

Immunocytochemistry

MDA-MB-231 cells

5% BSA, 0.3M glycine in PBST for 1 hour.

At 4° C overnight.

Fluorescent conjugated secondary antibody for 1 h at RT.

Nikon C2 confocal microscope.

PD-L1 and CMTM6 co-localise at the plasma membrane. PD-L1 knockout, CMTM6 knockout and parental MDA-MB-231 Cas9 cells were fixed, immunostained for PD-L1 and CMTM6 and analysed by confocal microscopy. Scale bar: 10μm. Please see Figure 2c in the article [1].

The same clone (MIH1) is sold as Invitrogen 13-5983-80, 13-5983-82, 14-5983-80, 14-5983-82, 16-5983-82, 17-5983-41, 17-5983-42, 25-5983-41, 25-5983-42, 48-5983-41, 48-5983-42, 47-5983-41, 61-5983-41, 61-5983-42, 47-5983-42, 16-5983-203; BD Biosciences 561787, 557924, 558065, 558017, 563740, 563741, 563742, 563739, 563738.