This is a knockout-validated antibody summary, based on the publication "A CD22-Shp1 phosphatase axis controls integrin β 7 display and B cell function in mucosal immunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1,κ
Company: BD Biosciences
Antibody: CD22.2
Catalog number: 553382
Summary: Mouse monoclonal antibody against B10.D2 mouse splenocytes. Reacts with mouse by flow cytometry, immunohistochemistry-frozen and immunoprecipitation (reported).
Immunocytochemistry
WT and Cd22–/– B cells isolated from mouse splenocytes.
In 1× PB for 60 min at 4°C.
Alexa Fluor 647-conjugated streptavidin.
Images were captured on a Zeiss LSM 880 confocal microscope using the Zeiss Zen software, a 63× oil-immersion objective and 1.5× digital zoom.
Figure: Immunofluorescent staining of cell surface (c) and intracellular (d) β7 and CD22 in B cells with nucleus (DAPI, blue); cytoplasm (MitoTracker™ Deep Red, Red); β7 (green) and CD22 (magenta). Scale bars: 2 μM. Please see Figure 2d in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- productgene