CD168 antibody | knockout validation | Abcam ab124729

This is a knockout-validated antibody summary, based on the publication "HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD168 antibody | knockout validation | Abcam ab124729 figure 1
Figure 1. HMMR expression in tissues extracted from WT, HMMR-Het, or HMMR-KO mice. Actin served as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CD168

Catalog number: ab124729

Summary: Rabbit monoclonal IgG against a synthetic peptide within human CD168 (HMMR ) aa 1-100. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin). Unsuitable for flow cytometry or immunocytochemistry.

Validation Method

Western blot

Sample

Spleen and testes tissues from WT, HMMR-Het, or HMMR-KO mice. Tissues were lysed in RIPA buffer supplemented with protease inhibitor (Roche, Switzerland).

Primary incubation

1:500 dilution.

Clone note

The same clone (EPR4054) is sold as Abcam ab124729; EMD Millipore MABT468.

References
  1. Connell M, Chen H, Jiang J, Kuan C, Fotovati A, Chu T, et al. HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.. elife. 2017;6: pubmed publisher