This is a knockout-validated antibody summary, based on the publication "CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG
Company: Bio-Rad
Antibody: CD163
Catalog number: MCA2311GA
Summary: Mouse monoclonal IgG against CD163 from porcine alveolar macrophages. Reacts with porcine by western blot, Immunoprecipitation, immunohistochemistry (frozen), immunofluorescence and flow cytometry.
Western blot
Lung tissues from CD163 KO pigs.
5% non-fat dry milk.
HRP-conjugated anti-mouse IgG (ab6789, Abcam).
Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific). Chemiluminescent signals were captured by a cooled charged-coupled device (CCD) camera.
Western blot showing the CD163 protein expression is undetectable in the lungs of KO pigs. Two antibodies that recognized non-reducing (upper panel) and reducing CD163 (middle panel) proteins, respectively, were used for detecting CD163, and β-actin was stained as loading control (lower panel). CD163 protein possessed a molecular mass of approximately 130 kDa. A higher-molecular-mass band of unknown identity was also detected in the lungs of pigs. Please see Figure 1c in the article [1].
The same clone (2A10/11) is sold as Bio-Rad MCA2311F, MCA2311PE, MCA2311GA; Invitrogen MA5-16476, MA5-28292, MA5-28293.
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