This is a knockout-validated antibody summary, based on the publication "Deletion of prominin-1 in mice results in disrupted photoreceptor outer segment protein homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rat monoclonal IgG1, kappa

Company: Thermo

Antibody: CD133 (Prominin-1)

Catalog number: 14-1331-82

Summary: Rat monoclonal antibody against mouse Prominin-1. Reacts with dog and mouse. Suitable for western blot, immunohistochemistry (paraffin and frozen), immunocytochemistry/immunofluorescence, immunoprecipitation and flow cytometry.

Western blot | Immunohistochemistry

WT and Prom1-KO mouse retinas.

IHC: PBS with 5% goat serum, 0.5% Tween20.

WB/IHC: 1:1,000 dilution overnight at 4°C.

WB: HRP-conjugated goat anti-rat .

IHC: Alexa Fluor 488/594 antibodies for 2h in room temperature.

WB: Chemiluminescent substrates and Amersham Imager 600 (GE, USA).

IHC: Confocal microscope (LEICA, USA).

Immunofluorescence analysis of Prom1-KO and WT mice with commercial Prom1 antibody; D: Absence of Prom1 protein in Prom1-KO mice retina compared with WT. β-tubulin was used as a loading control. ONL: Outer nuclear layer. Please see Figure 1C,D in the article [1].