CD13 antibody | knockout validation | Abcam ab108310

This is a knockout-validated antibody summary, based on the publication "CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

CD13 antibody | knockout validation | Abcam ab108310 figure 1
Figure 1. Detection of pAPN expression in different small intestine segments by IHC. PAMs and tissues were derived from DKO and WT pigs. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: CD13

Catalog number: ab108310

Summary: Rabbit monoclonal against a synthetic peptide corresponding to CD13 (APN). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry and immunohistochemistry (paraffin). Unsuitable for flow cytometry or immunoprecipitation.

Validation Method

Immunohistochemistry

Sample

WT and CD163/pAPN-dKO duodenum, jejunum, and ileum tissues.

Primary incubation

1:300 dilution.

Clone note

The same clone (EPR4058) is sold as OriGene TA307568; Abcam ab108310.

References
  1. Xu K, Zhou Y, Mu Y, Liu Z, Hou S, Xiong Y, et al. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance. elife. 2020;9: pubmed publisher