This is a knockout-validated antibody summary, based on the publication "Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of CBLB in whole‐cell lysates (WCLs) of CD4+ T cells isolated from WT or CBLB‐deficient mouse (Cblb −/−) and kept unstimulated (−) or stimulated for 1 min with anti‐CD3 and anti‐CD4 antibodies (+). CD5 served as loading control. Molecular masses are shown (kDa). From [1].

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: CBLB

Catalog number: sc-376409

Summary: Mouse monoclonal IgG2b (kappa light chain) specific for an epitope mapping between amino acids 953-982 at the C-terminus of Cbl-b of human origin. Recommended for detection of Cbl-b of human, mouse, and rat origin by western blot, immunoprecipitation, immunofluorescence, Immunohistochemistry (paraffin) and ELISA.

Western blot

CD4+ T cells isolated from WT or CBLB‐deficient mouse. Cells were lysed in buffer (100 mM Tris, pH 7.5, 270 mM NaCl, 1 mM EDTA, 20% glycerol, 0.2% n-dodecyl-b-maltoside) supplemented with protease and phosphatase inhibitors.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.