CBLB antibody | knockout validation | Abcam ab54362
This is a knockout-validated antibody summary, based on the publication "Tumor rejection in Cblb -/- mice depends on IL-9 and Th9 cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Western blot analysis of Cbl-b, IRF4 and PU.1 protein expression after 3 days of Th9 cells differentiation or sole TCR stimulation (CD3/28) of WT and Cblb−/− naïve T cells. Please see Figure XXXX in the article [1].
Antibody information
Mouse monoclonal IgG2a
Company: Abcam
Antibody: CBLB
Catalog number: ab54362
Summary: Mouse monoclonal antibody against a recombinant C terminal fragment of human CBLB. Reacts with mouse and human. Suitable for western blot and immunohistochemistry (paraffin).
Validation Method
Western blot
Sample
WT and Cblb−/− naïve T cells.
Primary incubation
1:1,000 dilution.
Clone note
The same clone (246C5a) is sold as LifeSpan Biosciences LS-C184529, LS-C342566; Abcam ab54362; MyBioSource MBS120301.
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