This is a knockout-validated antibody summary, based on the publication "A novel CBL-Bflox/flox mouse model allows tissue-selective fully conditional CBL/CBL-B double-knockout: CD4-Cre mediated CBL/CBL-B deletion occurs in both T-cells and hematopoietic stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western Blot validating the deletion of CBL-B in EIIA-Cre CBL-B floxed mice. Splenocytes were collected from mice with indicated genotype and total protein lysate were blotted for CBL-B and HSC70 (loading control). From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: CBL-B

Catalog number: 9498S

Summary: Rabbit monoclonal IgG a synthetic peptide corresponding to residues near the carboxy terminus of human Cbl-b protein. Reacts with human, mouse, rat, and monkey by western blot. It is predicted to react based on 100% sequence homology with bovine.

Western blot

Splenocytes from WT, CBL-B null, CBL-B floxed and EIIA-Cre CBL-B floxed mice. Cells were lysed using lysis buffer (1 M Tris pH 7.5, 5 M NaCl, 10% Triton, 100 mM VO4, 1 M NaF, 50 mM PMSF).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.