This is a knockout-validated antibody summary, based on the publication "Using oriented peptide array libraries to evaluate methylarginine-specific antibodies and arginine methyltransferase substrate motifs", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Figure 1 in [1]. Western blot of lysates of wild-type and CARM1 -/- mouse embryonic fibroblasts probed with anti-CARM1 antibody. Actin serves as a loading control.

rabbit polyclonal

Company: Bethyl Laboratories

Antibody: CARM1

Catalog number: A300-421A

Summary: Rabbit polyclonal antibody.

Supplier recommended for WB, IP, IF and IHC.

Epitope affinity-purified. Recognises an against an epitope between residue 550 and 585 of human CARM1.

Reacts with human and mouse CARM1.

Western blot

Lysates of wild-type and CARM1 -/- mouse embryonic fibroblasts.

PBS containing 5% milk powder and 0.1% Tween 20.

Anti-CARM1 antibody.

HRP-conjugated secondary antibody.

Chemiluminescence (Perkin Elmer).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.