This is a knockout-validated antibody summary, based on the publication "Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of CARD9 expression in WT and Card9−/− cell-sorted bone marrow-derived neutrophils. Actin was used as loading control, from figure 5 [1].

Antibody: Bcl10

Catalog number: sc-99054

Summary: Rabbit polyclonal IgG, against amino acids 98-187 mapping within an internal region of CARD 9 of human origin. Recommended for detection of CARD 9 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine.

Company: Santa Cruz Biotechnology

Western blot

Neutrophils were lysed in 100 mM NaCl, 30 mM Na-HEPES (pH 7.4), 20 mM NaF, 1 mM Na-EGTA, 1% Triton X-100, 1 mM benzamidine, freshly supplemented with 0.1 U ml−1 Aprotinin, 1:100 Mammalian Protease Inhibitor Cocktail, 1:100 Phosphatase Inhibitor Cocktail 2, 1 mM phenylmethylsulphonyl fluoride and 1 mM Na3VO4 (all from Sigma).

0.2 ug ml−1

peroxidase-labelled secondary antibodies (GE Healthcare; dilution: 1:5,000).

ECL system (GE Healthcare) and exposed to X-ray film.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.