This is a knockout-validated antibody summary, based on the publication "CARD9 knockout ameliorates myocardial dysfunction associated with high fat diet-induced obesity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: CARD9
Catalog number: ab124922
Summary: Rabbit monoclonal IgG against a synthetic peptide from human CARD9. Reacts with human, mouse and rat. Suitable for western blot and immunohistochemistry (paraffin). Unsuitable for flow cytometry, immunocytochemistry or immunoprecipitation.
Western blot
Heart tissue and peritoneal macrophages from WT and CARD9−/− mice. Samples were lysed in ice cold RIPA buffer.
Overnight at 4°C.
Enhanced chemiluminescence detection solution.
Western immunoblotting analyses of CARD9 and β-actin (loading control) in mouse heart (A) and macrophages (B) after 5-month normal diet (ND) or high fat diet (HFD) feeding in WT and CARD9−/− mice. Please see Figure 7a,b in the article [1].
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