This is a knockout-validated antibody summary, based on the publication "The molecular, temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemistry stainings of CAMK2B, showing (Top to bottom, left to right): normal expression in WT mice and no expression in Camk2bΔex2/Δex2 mice; normal expression in Camk2bf/fmice and deletion throughout the brain in Camk2bf/f;CAG-CreERmice. From [1].

Rabbit polyclonal IgG

Company: abcam

Antibody: CAMK2B

Catalog number: ab34703

Summary: Rabbit polyclonal IgG against a synthetic peptide derived from within residues 350 - 450 of mouse CaMKII beta. Reacts with mouse and rat. Suitable for western blot, immunohistochemistry (frozen and paraffin), immunocytochemistry/immunofluorescence.

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Figure 2. (a) Immunohistochemistry stainings of CAMK2B, showing (left to right): normal expression in Camk2bf/f mice; specific deletion in cerebellar Purkinje cells in Camk2bf/f;L7-cre mice and specific deletion in the cerebellar granule cells of Camk2bf/f;Gabaa6-cre mice. (b) Immunofluorescent and immunohistochemical zoomed-in picture of the cerebellum of Camk2bf/fmice, Camk2bf/f;L7-cre and Camk2bf/f;Gabaa6-cre mice showing specific deletion in cerebellar Purkinje Cells in the Camk2bf/f;L7-cre mice and deletion in the granule cell layer (GCL) of Camk2bf/f;Gabaa6-cre mice, but not in Purkinje cells of the molecular layer (ML). From [1].

Immunohistochemistry

Brains from control Camk2bf/f and conditional cell-specific Camk2b null mice. Brains were fixed in PFA and 40μm thick sagittal cryostat sections were processed as free-floating.

IHC: 1:2,000 dilution in PBS containing 2% NHS, 0.5% Triton-X 100 and 150 mM bovine serum albumin (BSA) and kept at 4 °C overnight for 48 hours.

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Figure 3. Immunohistochemistry stainings of CAMK2B, showing (left to right): normal expression in Camk2bf/fmice; specific deletion in the striatum of Camk2bf/f;Rgs9-cre and specific hippocampal and cortical deletion in Camk2bf/f;Emx-cre mice. From [1].

IF: 1:1,000 dilution.

IHC: 1:200 dilution biotinylated goat anti-rabbit IgG antibody (Vector Laboratories, Burlinghame, CA) in PBS containing 2% NHS, 0.5% Triton-X 100 and 150 mM bovine serum albumin (BSA) for 1 to 2 hours on room temperature.

IF: 1:200 dilution Cy3 rabbit secondary antibody 1 to 2 hours at room temperature.

IHC: Standard avidin-biotin-immunoperoxidase complex method (ABC, Vector Laboratories, USA) and 0.05% DAB as the chromogen.