This is a knockout-validated antibody summary, based on the publication "Epithelial-mesenchymal transition leads to NK cell-mediated metastasis-specific immunosurveillance in lung cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western immunoblot validation of CADM1 deletion utilizing two separate antibodies. 3E1 recognizes the N-Terminal of CADM1 while SIGMA recognizes the C-Terminal domain. Please see Suppl. Figure 6b in the article [1].

Chicken monoclonal IgY

Company: MBL International

Antibody: CAM (TSLC1/CADM1)

Catalog number: CM004-3

Summary: Chicken monoclonal IgY against a recombinant fusion protein Fc-SynCAM. Reacts with human, and mouse. Suitable for western blot, immunoprecipitation, immunohistochemistry and flow cytometry.

Western blot

Cells were washed with DPBS, pH 7.4, after treatment and lysed in radioimmunoprecipitation assay buffer (RIPA) containing NaF, Na3VO4, and protease inhibitor.

1:1,000 dilution.

1:10,000 dilution anti-chicken–HRP (polyclonal; catalog 31401, Thermo Fisher Scientific).