This is a knockout-validated antibody summary, based on the publication "Exploiting a novel conformational switch to control innate immunity mediated by complement protein C3a", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot showing no human C3aR expression in wild-type human HEK293 cells (C3aR^−/−) compared to the same cells transfected with C3aR (C3aR+/+). From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: C3aR

Catalog number: sc-133172

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 183-482 mapping at the C-terminus of human C3aR. Recommended for detection of C3aR of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Wild-type (C3aR^−/−) and C3aR-transfected (C3aR+/+) human HEK293 cells.

1:200 dilution.

1:500 dilution HRP-linked anti-mouse IgG (Cell Signaling Technology, #7076).

Wild-type HEK293 cells (C3aR^−/−) do not express the C3a receptor. The experiment is not a KO, but the receptor is transfected into the cells. Technically is not a KO, although mAb might be considered validated.