This is a knockout-validated antibody summary, based on the publications "Astrocyte-Microglia Cross Talk through Complement Activation Modulates Amyloid Pathology in Mouse Models of Alzheimer's Disease", as cited below [1] and “Microglia Exacerbate White Matter Injury via Complement C3/C3aR Pathway After Hypoperfusion” for immunohistochemistry (figure 5a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Hycult Biotech
Antibody: C3aR
Catalog number: HM1123
Summary: Rat monoclonal IgG2a against RBL-2H3 transfectants expressing mouse C3aR. Reacts with mouse by flow cytometry and immunohistochemistry (frozen).
Immunohistochemistry
Brain sections from WT and C3aR KO mice. Mice were perfused with 4% PFA, and the brains were post-fixed in 4% PFA, cryoprotected and sectioned.
PBST containing 3% BSA and 2% donkey serum for 30 min.
Primary antibody diluted in blocking solution overnight at 4°C.
Secondary antibody for 1 h at room temperature.
Leica laser confocal microscope.
Please see Figure 1d in the article [1].
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