This is a knockout-validated antibody summary, based on the publication "HID-1 is required for homotypic fusion of immature secretory granules during maturation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot analysis of mouse HID-1 and actin in isolated islets from WT and Hid1-betaKO (KO) mice. From [1].

Mouse monoclonal IgG1

Company: Origene

Antibody: C17orf28

Catalog number: TA501311

Summary: Mouse monoclonal IgG1 against full length human recombinant protein of human C17orf28. Reacts with human, dog, rat, and monkey. Suitable for western blot, immunohistochemistry, immunofluorescence and flow cytometry.

[enlarge]

Figure 2. Immunofluorescent histochemical analysis of pancreas sections of WT and KO mice, showing glucagon (green), insulin (red) and HID-1 (cyan). Scale bar: 20 μm. From [1].

Western blot | Immunohistochemistry

WB: Islet samples were lysed in standard RIPA buffer (150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, and 50 mM Tris, pH 8.0). IHC: Pancreases were dissected from paired WT and Hid1-betaKO mice and fixed with 4% paraformaldehyde in PBS for 15 min.

IHC: 5% goat serum in PBS for 60 min.

IHC: primary antibodies diluted in PBS containing 2.5% goat serum for 60 min.

IHC: Alexa Fluor 405-conjugated goat anti-mouse (Thermo Fisher, Waltham, MA) for 60 min at 37˚C.

IHC: Olympus FV1200 Laser Scanning Confocal Microscope (Olympus, Tokyo, Japan) with a 60x (NA = 1.40) oil objective.

Rabbit polyclonal anti-HID-1 (Proteintech, 21174-1-AP) is KO validated in Fig 3E.