This is a knockout-validated antibody summary, based on the publication "BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. MEFs of indicated genotypes were stained for BubR1 (red), centromeres (cyan), and DNA (blue). WT, wild-type. FL, full-length. From [1].

Mouse monoclonal IgG2a

Company: BD Transduction

Antibody: BubR1

Catalog number: 612503

Summary: Mouse monoclonal IgG2a against human BUBR1 aa. 276-388. Reacts with human by western blot and immunofluorescence.

Immunocytochemistry

WT and different domain deletion BubR1 MEFs. Cells were fixed in 3% paraformaldehyde.

PBS–2% nonfat milk (Kroger Co., Cincinnati, Ohio).

1:250 dilution overnight in blocking buffer.

Texas red-conjugated goat anti-mouse antibodies at 4 μg per ml in blocking buffer.

Laser-scanning microscope (LSM 880; Carl Zeiss) with an inverted microscope (Axiovert 100 M; Carl Zeiss).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.