Bmi1 antibody | knockout validation | Abcam ab126783

This is a knockout-validated antibody summary, based on the publication "BMI1 activates P-glycoprotein via transcription repression of miR-3682-3p and enhances chemoresistance of bladder cancer cell", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Bmi1 antibody | knockout validation | Abcam ab126783 figure 1
Figure 1. Western Blot detection of BMI1 protein in T24/DDP&GEM cells upon BMI1 knockout using CRISPR/Cas9 system. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Bmi1

Catalog number: ab126783

Summary: Rabbit monoclonal antibody against a synthetic peptide of Bmi1. Reacts with rat and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and ChIC/CUT&RUN-seq.

Validation Method

Western blot

Sample

Control and BMI1 KO T24/DDP&GEM cells.

Clone note

The same clone (EPR3745(2)) is sold as LifeSpan Biosciences LS-B8010; Abcam ab126783.

References
  1. Chen M, Zhou J, Wang P, Ye Y, Liu Y, Zhou J, et al. BMI1 activates P-glycoprotein via transcription repression of miR-3682-3p and enhances chemoresistance of bladder cancer cell. Aging (Albany NY). 2021;13:18310-18330 pubmed publisher