This is a knockout-validated antibody summary, based on the publication "Electron transport chain activity is a predictor and target for venetoclax sensitivity in multiple myeloma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Company: Cell Signaling
Antibody: Bim
Catalog number: 2933
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro25 of Bim. Reacts with human, mouse, and rat. It is predicted to react based on 100% sequence homology with monkey, bovine, dog, and pig. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and Leica Bond), immunofluorescence/immunocytochemistry and flow cytometry.
Western blot
Control and BIM-KO L363 and KMS11 cells.
5% milk in tris-buffered saline with tween TBST) for 1 h.
1:1,000 dilution made in BLOK™ Casein in TBS (G-Biosciences, #786-196) overnight.
3:10,000 in blocking solution for 1 h.
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