This is a knockout-validated antibody summary, based on the publication "Postnatal Development of Spasticity Following Transgene Insertion in the Mouse βIV Spectrin Gene (SPTBN4)", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2bκ
Company: Merck Millipore
Antibody: Beta-IV spectrin
Catalog number: MABN1727
Summary: Mouse monoclonal IgG2bκ against a recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin. Reacts with human and rat. It is predicted to react based on 100% sequence homology with mouse. Suitable for western blot and immunohistochemistry.
Western blot
Brain lysates from wildtype, SPTBN4+/– (L25+/–) and SPTBN4–/– (L25+/+) mice.
Representative western blot of brain lysates from wildtype (WT), unaffected transgenic (L25+/–) or affected transgenic (L25+/+) mice probed for IV spectrin. Wild-type brain lysates reveal strong labelling for IV spectrin, while brains of the affected (L25+/+) mice showed no bands. Diminished bands are evident for L25+/– mouse brains. GAPDH labeling of the same samples demonstrated equal loading and transfer of protein. Please see Figure 3 in the article [1].
The same clone (N393/76) is sold as Neuromab 73-377, 75-377; EMD Millipore MABN1727.
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