Bcl10 antibody | knockout validation | Cell Signaling 4237

This is a knockout-validated antibody summary, based on publications "Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells", as cited below [1], and "TRIM41 is required to innate antiviral response by polyubiquitinating BCL10 and recruiting NEMO" for western blot knockout validation (figure 6c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Bcl10 antibody | knockout validation | Cell Signaling 4237 figure 1
Figure 1. Immunoblot of sorted splenic CD4+ and CD8+ T cells as well as splenic B220+ B cells of either Bcl10fl/fl or Bcl10fl/fl;CD4-Cre mice to detect the protein expression of BCL10. β-actin was used as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Bcl10

Catalog number: 4237

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ser60 of Bcl10. Reacts with human, mouse, and rat. It is predicted to react based on 100% sequence homology with monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Bcl10-KO splenic mouse lymphocytes.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:3,000 dilution anti-rabbit IgG-HRP (Cell Signaling).

References
  1. Rosenbaum M, Gewies A, Pechloff K, Heuser C, Engleitner T, Gehring T, et al. Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells. Nat Commun. 2019;10:2352 pubmed publisher
  2. Yu Z, Li X, Yang M, Huang J, Fang Q, Jia J, et al. TRIM41 is required to innate antiviral response by polyubiquitinating BCL10 and recruiting NEMO. Signal Transduct Target Ther. 2021;6:90 pubmed publisher