Bcl-w antibody | knockout validation | Cell Signaling 2724

This is a knockout-validated antibody summary, based on the publication "BCL-W has a fundamental role in B cell survival and lymphomagenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Bcl-w

Catalog number: 2724

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding alanine 39 of Bcl-w. Reacts with human, mouse and rat by western blot.

Validation Method

Western blot

Sample

Bone marrow-derived pre–B cells from Bcl-w+/+, Bcl-w+/–, and Bcl-w–/- mice.

Figure

Western blotting of BCL-W and β-actin (loading control) in bone marrow-derived pre–B cells from Bcl-w+/+, Bcl-w+/–, and Bcl-w–/- mice. Please see Figure 1 in the article [1].

Note

p53 (Ab-7; Calbiochem) is also KO validated (Figure 3c).

References
  1. Adams C, Kim A, Mitra R, Choi J, Gong J, Eischen C. BCL-W has a fundamental role in B cell survival and lymphomagenesis.. J Clin Invest. 2017;127:635-650 pubmed publisher