This is a knockout-validated antibody summary, based on the publication "Reciprocal negative regulation between the tumor suppressor protein p53 and B cell CLL/lymphoma 6 (BCL6) via control of caspase-1 expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG3 (kappa light chain)
Company: Santa Cruz
Antibody: Bcl-6
Catalog number: sc-7388
Summary: Mouse monoclonal IgG3 (kappa light chain) against amino acids 3-484 mapping at the N-terminus of human Bcl-6. Recommended for detection of Bcl-6 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).
Western blot
Cells were harvested and lysed in RIPA buffer (50 mM Tris-HCl pH 8.0, 1% NP-40,
0.25% sodium deoxycholic acid, 150 mM NaCl, 1 mM EGTA, and complete MiniProtease cocktail).
5% skim milk (BD Biosciences).
Anti-mouse secondary antibodies conjugated to HRP (Vector Laboratories).
Protein bands were visualized with ECL solution (PerkinElmer).
Western blot analyses of the indicated proteins in spleen tissues from Bcl6 WT and Bcl6 KO mice. Please see Figure 3i in the article [1].
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