Bax antibody | knockout validation | Cell Signaling 5023

This is a knockout-validated antibody summary, based on the publication "Blocking downstream signaling pathways in the context of HDAC inhibition promotes apoptosis preferentially in cells harboring mutant Ras", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Bax antibody | knockout validation | Cell Signaling 5023 figure 1
Figure 1. Western blot of Bak and Bax in parental HCT-116 cells or cells lacking Bak (Bak−/−), Bax (Bax−/−) or both proteins (double knock-out, DKO). GAPDH served as a loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Bax

Catalog number: 5023

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu45 of human Bax protein. Reacts with human by western blot, immunoprecipitation and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Wildtype, Bax−/− and Bak/Bax double knock-out HCT-116 cells.

Secondary incubation

1:10,000 dilution of IRDye secondary antibodies (LI-COR).

Detection

Odyssey System (LI-COR, Lincoln, NE).

References
  1. Bahr J, Robey R, Luchenko V, Basseville A, Chakraborty A, Kozlowski H, et al. Blocking downstream signaling pathways in the context of HDAC inhibition promotes apoptosis preferentially in cells harboring mutant Ras. Oncotarget. 2016;7:69804-69815 pubmed publisher