This is a knockout-validated antibody summary, based on the publication "Subcellular localization of PUMA regulates its pro-apoptotic activity in Burkitt's lymphoma B cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure S5 in [1]. Western blot of lysates of mouse embryonic fibroblasts from wild-type, Bak -/- and Bak/BAX double knockout animals probed with anti-Bak antibody. GAPDH serves as a loading control.

Rabbit polyclonal

Company: Cell Signaling Technology

Antibody: Bak

Catalog number: 38145 (Should this be 3814S?)

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to residues surrounding Gly82 of human Bak.

Supplier recommended for WB.

Reacts with human, mouse, rat and monkey Bak.

Western blot

Lysates of mouse embryonic fibroblasts from wild-type, Bak -/- and Bak/BAX double knockout animals.

Anti-Bak antibody.

Immobilon West chemiluminescent substrate (Merck Millipore).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.