This is a knockout-validated antibody summary, based on the publication "Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot validation results for pro-apoptotic Bcl-2 family member Bak. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Bak

Catalog number: ab32371

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human Bak (aa 1-100). Reacts with human. Suitable for western blot, immunohistochemistry (paraffin), flow cytometry and immunocytochemistry/immunofluorescence.

Figure 2. Immunocytochemistry validation results for pro-apoptotic Bcl-2 family member Bak. From [1].

Western blot | Immunocytochemistry

Control and (Bax/Bak)-/- HCT-116 cells.

5% non-fat dry milk in Tris-buffered saline with Tween 20 (TBST) (15 mm Tris-HCl, pH 7.5, 200 mm NaCl, and 0.1% Tween 20) at room temperature for 1 h.

At room temperature for 2 h or overnight at 4˚C.

Peroxidase-conjugated secondary antibodies (Jackson Laboratories) for 1 h.

Enhanced chemiluminescence detection reagent (Millipore, Ireland).