This is a knockout-validated antibody summary, based on the publication "BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Bad
Catalog number: ab32445
Summary: Rabbit monoclonal antibody against a synthetic peptide within human Bad (N terminal). Reacts with mouse, rat, adn human. It is predicted to work with dog. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry (intra).
Western blot
WT and Bad-KO mouse joint extracts.
5% (w/v) skim milk diluted in TBST for 1 hr at room temperature.
HRP-linked anti-rabbit IgG (#7074, CST) for 1 hr at room temperature.
Enhanced chemiluminescence method.
The same clone (Y208) is sold as OriGene TA300475; Abcam ab32445, ab220116.
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