BRD4 antibody | knockout validation | Cell Signaling 13440S

This is a knockout-validated antibody summary, based on the publication "An Argonaute phosphorylation cycle promotes microRNA-mediated silencing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: BRD4

Catalog number: 13440S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu1129 of human BRD4 protein. Reacts with human and it is predicted to react based on 100% sequence homology with bovine, dog, and pig. Suitable for western blot, immunoprecipitation (IP), chromatin IP and Chromatin IP-seq.

Validation Method

Western blot

Sample

Wildtype and BRD4 knockout HCT116 cells.

Figure

Western blot analysis confirming loss of expression of BRD4 in HCT116 knockout clones. Please see Extended Data Figure 2b in the article [1].

References

Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;: pubmed publisher

contribute

If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.