BRCA1 antibody | knockout validation | ThermoFisher Scientific MA1-23164
DOI
//dx.doi.org/10.13070/ko.en.7.1934
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "Analysis of alternative lengthening of telomere markers in BRCA1 defective cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: ThermoFisher Scientific

Antibody: BRCA1

Catalog number: MA1-23164

Summary: Mouse monoclonal antibody raised against and E.coli-expressed polypeptide corresponding to residues 340-748 of BRCA1.

Supplier recommended for WB, IP, IF, ICC, IHC and ChIP.

Reacts with mouse and human BRCA1.

Validation Method

Western blot

Immunocytochemistry

Sample

Western blot: lysates of wild-type (E14) and BRCA1 knockout (E408) mouse embryonic stem cells.

Immunocytochemistry: wild-type (E14) and BRCA1 knockout (E408) mouse embryonic stem cells.

Blocking agent

Western blot: not stated.

Immunocytochemistry: PBS containing 0.2% cold water fish gelatin (Sigma G-7765) and 0.5% BSA (Sigma A-2153).

Primary incubation

Western blot: antiBRCA1 diluted 1:1000.

Immunocytochemistry: anti-BRCA1 diluted 1:400.

Secondary incubation

Western blot: HRP-conjugated anti-mouse IgG (diluted 1:500).

Immunocytochemistry: anti-mouse IgG diluted (1:100).

Figure

See Figures 4 and Supplementary Figure S3 in [1].

Clone note

The same clone (6B4) is sold as Bio-Rad MCA5945GT; LifeSpan Biosciences LS-C19935; Invitrogen MA1-23164; GeneTex GTX70115.

References
  1. Kargaran P, Yasaei H, Anjomani-Virmouni S, Mangiapane G, Slijepcevic P. Analysis of Alternative Lengthening of Telomere Markers in BRCA1 Defective Cells. Genes Chromosomes Cancer. 2016;: pubmed publisher