This is a knockout-validated antibody summary, based on the publication "Transfer of malignant trait to BRCA1 deficient human fibroblasts following exposure to serum of cancer patients", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of proteins extracted from control fibroblasts (empty vector transfected) and BRCA1-KO fibroblasts. β-Actin serves as loading control. Note that BRCA1 signal is absent in BRCA1-KO fibroblasts. (*) points to the BRCA1 signal. From [1]. This antibody seems not so specific. WB is not very clean.

Rabbit polyclonal IgG

Company: Abcam

Antibody: BRCA1

Catalog number: ab131360

Summary: Rabbit polyclonal IgG against a synthetic peptide from around amino acids 1522-1526 of human BRCA1. Reacts with human by western blot and immunocytochemistry/immunofluorescence.

Western blot

Control (empty vector transfected) and BRCA1-KO human fibroblasts. Cells were lysed in RIPA buffer containing protease inhibitors (Sigma, Oakville, Canada).

TBS containing 5 % non-fat dry milk.

Overnight at 4 °C.

Anti-rabbit peroxidase-conjugated secondary antibody for 1 h at room temperature.

Immobilon Western HRP Substrate (Millipore, Etobicoke, Canada).