This is a knockout-validated antibody summary, based on the publication "Epidermal RAF prevents allergic skin disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 6 in [1]. Western blot of lysates of control epidermal cells and epidermal cells with BRAF knockout or BRAF/RAF1 double knockout. Probed with anti-BRAF antibody. ACTB serves as a loading control.

Mouse monoclonal

Company: Santa Cruz Biotechnology

Antibody: BRAF

Catalog number: sc-5284

Summary: Mouse monoclonal antibody raised against residues 12-133 of human BRAF.

Supplier recommended for WB, IP, IF, IHC and elisa.

Reacts with human, mouse and rat BRAF.

Western blot

Lysates of control epidermal cells and epidermal cells with BRAF knockout or BRAF/RAF1 double knockout.

Anti-BRAF antibody.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.