BNIP3 antibody | knockout validation | Abcam ab10433

This is a knockout-validated antibody summary, based on the publication "Activation of BNIP3-mediated Mitophagy Protects Against Renal Ischemia-Reperfusion Injury", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

BNIP3 antibody | knockout validation | Abcam ab10433 figure 1
Figure 1. Representative images of BNIP3 staining in kidney tissues of Bnip3 deficiency (KO) mice and their wild-type littermates (WT) (male, 8 weeks old), subjected to 30 min bilateral renal ischemia followed by 48 h of reperfusion (IR) or sham operation (sham). From [1].
Antibody information

Mouse monoclonal IgG2b, kappa light chain

Company: Abcam

Antibody: BNIP3

Catalog number: ab10433

Summary: Mouse monoclonal IgG2b against an epitope within amino acids 112-124 of human BNIP3. Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunoprecipitation, immunohistochemistry (paraffin), flow cytometry and ELISA.

Validation Method

Immunohistochemistry

Sample

WT and Bnip3-KO mouse kidneys.

Blocking agent

3% H2O2, 5% normal donkey serum and 0.1% Triton X-100

Primary incubation

1:100 dilution at 4 °C overnight.

Secondary incubation

Horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h at room temperature.

Detection

Signals of the antigen–antibody complexes were detected with a DAB Peroxidase Substrate Kit (Vector Laboratories) following the manufacturer’s introduction.

Clone note

The same clone (ANa40) is sold as Santa Cruz Biotechnology sc-56167; Abcam ab10433, ab196706; Invitrogen MA1-24688; MilliporeSigma B7931.

References
  1. Tang C, Han H, Liu Z, Liu Y, Yin L, Cai J, et al. Activation of BNIP3-mediated mitophagy protects against renal ischemia-reperfusion injury. Cell Death Dis. 2019;10:677 pubmed publisher