BMAL1 antibody | knockout validation | Santa Cruz sc-365645

This is a knockout-validated antibody summary, based on the publication "Bmal1 integrates mitochondrial metabolism and macrophage activation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

BMAL1 antibody | knockout validation | Santa Cruz sc-365645 figure 1
Figure 1. Immunoblots of Hif-1α and Bmal1 protein levels in WT and M-BKO macrophages during a 24-hr time course of M1 activation. From [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: BMAL1

Catalog number: sc-365645

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 441-610 mapping near the C-terminus of human BMAL1. Recommended for detection of BMAL1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and Bmal1-knockout (M-BKO) macrophages.

Primary incubation

1:1,000 dilution in TBST buffer with 1% BSA overnight.

Detection

BioRad ChemiDoc XRS+ imaging system.

References
  1. Alexander R, Liou Y, Knudsen N, Starost K, Xu C, Hyde A, et al. Bmal1 integrates mitochondrial metabolism and macrophage activation. elife. 2020;9: pubmed publisher