BECN1 antibody | knockout validation | Cell Signaling Technology 3495

This is a knockout-validated antibody summary, based on the publications "Divergent roles of BECN1 in LC3 lipidation and autophagosomal function" (discussed below) [1] and "p53-dependent autophagic degradation of TET2 modulates cancer therapeutic resistance" (see figure 5b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

BECN1 antibody | knockout validation | Cell Signaling Technology 3495 figure 1
Figure 1. Western blotting of cell lysates from WT and Beclin-1 -/- HeLa cell lysates. From [1].
Antibody information

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: Beclin-1

Catalog number: 3495 CloneD40C5

Summary: Raised against a peptide corresponding to residues surrounding Thr72 of human Beclin-1. It detects human, mouse, rat and monkey Beclin-1. Supplier recommended for Western blotting and immunoprecipitation.

Supplier recommended dilution for Western blotting is 1:1000.

This antibody recognises the N-terminal portion of Beclin-1.

Validation Method

Western blotting

Sample

Wild-type and Beclin-1 -/-HeLa cells lysates.

Primary incubation

Anti-beclin-1

Secondary incubation

HRP-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch; 111-035-003)

Incubation conditions not stated.

References
  1. He R, Peng J, Yuan P, Xu F, Wei W. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy. 2015;11:740-7 pubmed publisher
  2. Zhang J, Tan P, Guo L, Gong J, Ma J, Li J, et al. p53-dependent autophagic degradation of TET2 modulates cancer therapeutic resistance. Oncogene. 2018;: pubmed publisher