This is a knockout-validated antibody summary, based on the publication "Divergent roles of BECN1 in LC3 lipidation and autophagosomal function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting of cell lysates from WT and Beclin-1 -/- HeLa cell lysates. Middle lanes (BECN1-C) show membrane probed with BD Transduction Laboratories anti-Beclin antibody (Cat. No. 612112). TUBB served as a loading control. BECN1-N is Cell Signaling Technology 3495. From [1].

Mouse monoclonal

Company: BD Transduction Laboratories

Antibody: Beclin

Catalog number: 612112

Summary: Raised against a peptide corresponding to residues 171-291 of human beclin. It detects human, mouse, rat, chicken and dog Beclin. Supplier recommended for Western blotting.

Note: supplier recommends against use in immunofluorescence applications.

Supplier recommended dilution for Western blotting is 1:500 – 1:2000.

This antibody recognises the C-terminal portion of beclin.

Western blotting

Wild-type and Beclin-1 -/-HeLa cell lysates.

Anti-beclin.

HRP-conjugated goat anti-mouse IgG (Jackson ImmunoResearch; 115-035-003)

Incubation conditions not stated.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.